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Open Access Research article

Genetic effects of polymorphisms in candidate genes and the QTL region on chicken age at first egg

Haiping Xu1, Hua Zeng1, Chenglong Luo2, Dexiang Zhang1, Qian Wang1, Liang Sun1, Lishan Yang1, Min Zhou3, Qinghua Nie1 and Xiquan Zhang1*

Author Affiliations

1 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, Guangdong, China

2 Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong, China

3 Biotechnology Institute, Jiang Xi Education College, Nanchang 330029, Jiangxi, China

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BMC Genetics 2011, 12:33  doi:10.1186/1471-2156-12-33

Published: 15 April 2011

Abstract

Background

The age at first egg (AFE), an important indicator for sexual maturation in female chickens, is controlled by polygenes. Based on our knowledge of reproductive physiology, 6 genes including gonadotrophin releasing hormone-I (GnRH-I), neuropeptide Y (NPY), dopamine D2 receptor (DRD2), vasoactive intestinal polypeptide (VIP), VIP receptor-1 (VIPR-1), and prolactin (PRL), were selected as candidates for influencing AFE. Additionally, the region between ADL0201 and MCW0241 of chromosome Z was chosen as the candidate QTL region according to some QTL databases. The objective of the present study was to investigate the effects of mutations in candidate genes and the QTL region on chicken AFE.

Results

Marker-trait association analysis of 8 mutations in those 6 genes in a Chinese native population found a highly significant association (P < 0.01) between G840327C of the GnRH-I gene with AFE, and it remained significant even with Bonferroni correction. Based on the results of the 2-tailed χ2 test, mutations T32742394C, T32742468C, G32742603A, and C33379782T in the candidate QTL region of chromosome Z were selected for marker-trait association analysis. The haplotypes of T32742394C and T32742468C were significantly associated (P < 0.05) with AFE. Bioinformatics analysis indicated that T32742394C and T32742468C were located in the intron region of the SH3-domain GRB2-like 2 (SH3GL2) gene, which appeared to be associated in the endocytosis and development of the oocyte.

Conclusion

This study found that G840327C of the GnRH-I gene and the haplotypes of T32742394C-T32742468C of the SH3GL2 gene were associated with the chicken AFE.