The response of NCF1Ψ/NCF1 gene expression ratio to macrophage differentiation. a) Pyrosequencing was carried out to measure the ratio of NCF1B, NCF1 and NCF1C copies in the genomic DNA of THP-1 and differentiated macrophages. b) RT-qPCR was performed to measure the NCF1Ψ/NCF1 gene expression level using primers that recognize the GT or GTGT containing transcripts from all three gene copies. Five independent experiments were performed in duplicates using both GAPDH and β-actin as a reference. P values are indicated. c) Pyrosequencing was carried out to measure the relative contributions of NCF1 and its pseudogenes to the GTGT or GT containing transcript pool during macrophage differentiation.
Brunson et al. BMC Genetics 2010 11:13 doi:10.1186/1471-2156-11-13