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Open Access Research article

A single mutation results in diploid gamete formation and parthenogenesis in a Drosophila yemanuclein-alpha meiosis I defective mutant

Régis E Meyer14, Michèle Delaage2, Roland Rosset2, Michèle Capri12 and Ounissa Aït-Ahmed123*

Author Affiliations

1 Institut de Génétique Humaine (IGH), Unité Propre de Recherche 1142, Centre National de la Recherche Scientifique (CNRS), 141 Rue de la Cardonille, 34396 Montpellier cedex 5, France

2 Previous address: IBDML, Campus de Luminy Case 907, 13288 Marseille, Cedex 09, France

3 Previous address: Department of Biological Sciences, Stanford University, Stanford, CA94305, USA

4 Current Address: Cell Cycle and Cancer Biology Oklahoma Medical Research Foundation 825 N.E. 13th Street Oklahoma City, Oklahoma 73104 USA

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BMC Genetics 2010, 11:104  doi:10.1186/1471-2156-11-104

Published: 16 November 2010

Additional files

Additional file 1:

Additional file 1 contains Additional Methods, 4 Additional Tables and Additional References.

Additional Methods

Genetic Methods

Genetic screens for yem-alpha mutant alleles

Rescue construct and germ line transformation

Sequencing of the yem-alpha mutant allele

Additional Tables

Table S1: Sequences used in the Clustal-W alignments

Table S2: Recombination defective genotypes used in this work (called mut-rec)

Table S3: Fertility rate and X chromosome segregation for various maternal genotypes.

Table S4: Drosophila stocks used in the work described in the main text

Additional References

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Open Data