Figure 3.

The number of cells in wild-type and out^m233mutant retinae. (A - B) Hoechst staining of 4-μm plastic sections through the retina of wild-type (A) and out^m233 (B) embryos at 36 hpf. (C) Diagram showing the number of nuclei that were counted in the dorsal, ventral, and entire retina. At 36 hpf, cell numbers in mutant embryos are not significantly different from these in wild-type siblings. Cells were counted on sections from 7 and 4 embryos for wild-type and mutant, respectively. (D - E) Hoechst staining of 4-μm plastic sections through the retina of wild-type (D) and out^m233 (E) embryos at 96 hpf. (F) Diagram showing the number of nuclei that were counted in the ganglion cell layer (GCL), in the inner nuclear layer (INL) and in the photoreceptor layer (PRCL). By 96 hpf, cell numbers in all these three layers are reduced in mutant embryos, compared to the wild type (for GCL, p < 0.01; for INL and PRCL, p < 0.001; t-test). For both wild-type and mutant, cells were counted on sections from 6 embryos. Asterisks indicate the lens, and arrowheads the optic nerve.