Table 1

Genetic variability at 25 microsatellites for 37 leopards (blood samples)

Locus

Repeat nature

Dye

Product size range

(bp)

Ta

No. of alleles

He

Ho

PID(unrelated) cumulative

PID(sibs) cumulative

Amplification success (%) for faeces

Allelic dropout

(%)

Multiplex sets for PCR


FCA126*$

Di

6-FAM

134 - 154

57.0

10

0.85

0.74

3.975E-02

3.356E-01

94%

0

Set 1

FCA279*$

Di

NED

81 - 105

57.0

11

0.83

0.68

1.882E-03

1.156E-01

88%

1.5

FCA304*$

Di

NED

105 - 129

58.0

8

0.80

0.37

1.255E-04

4.229E-02

88%

0

Single reaction

FCA052*$

Di

6-FAM

107 - 121

55.0

8

0.74

0.50

8.721E-08

2.523E-03

92.1%

1.5

Set 2

FCA090*$

Di

HEX

104 - 116

55.0

6

0.74

0.71

8.819E-10

4.058E-04

77%

3

FCA672*$

Di

6-FAM

82 - 100

54.0

8

0.74

0.68

8.564E-12

6.655E-05

92.1%

0

Single reaction

FCA230*$

Di

HEX

98 - 106

52.0

5

0.70

0.71

1.572E-15

2.098E-06

90%

6

Single reaction

FCA309*$

Di

6-FAM

92 - 106

55.0

6

0.67

0.58

8.754E-19

8.788E-08

94%

0

Single reaction

FCA205*

Di

NED

89 - 113

55.5

9

0.76

0.71

1.108E-05

1.646E-02

22%

3

FCA628*

Di

HEX

98 - 110

58.0

7

0.73

0.79

8.045E-11

1.638E-04

48%

9

F42

Tetra

NED

226 - 250

55.4

7

0.77

0.68

9.204E-07

6.404E-03

FCA391

Tetra

HEX

190 - 218

56.0

8

0.76

0.79

8.920E-09

1.006E-03

6HDZ056

Di

HEX

169 - 177

58.0

5

0.63

0.53

9.242E-13

2.709E-05

F115

Tetra

HEX

190 - 206

55.5

5

0.75

0.32

9.552E-14

1.106E-05

FCA506

Di

6-FAM

192 - 226

54.0

12

0.72

0.68

1.285E-14

4.786E-06

F41

Tetra

NED

123 - 139

56.0

5

0.68

0.68

2.381E-16

9.303E-07

6HDZ170

Di

6-FAM

212 - 226

60.0

6

0.69

0.61

3.581E-17

4.152E-07

FCA001

Di

6-FAM

139 - 163

54.0

10

0.68

0.66

5.968E-18

1.897E-07

FCA453

Tetra

6-FAM

181-201

59.0

4

0.64

0.55

1.643E-19

4.181E-08

6HDZ007

Di

HEX

202 - 226

65.0

10

0.65

0.50

3.022E-20

2.018E-08

E21B

Di

6-FAM

157 - 167

60.0

4

0.58

0.58

7.733E-21

1.063E-08

FCA232

Di

6-FAM

113 - 123

55.0

5

0.55

0.71

2.046E-21

5.749E-09

FCA441

Tetra

HEX

137 - 153

50.0

5

0.53

0.50

5.034E-22

3.129E-09

FCA164

Di

6-FAM

80 - 90

58.0

4

0.10

0.05

4.073E-22

2.821E-09

FCA170

Di

HEX

84 - 94

55.0

4

0.10

0.05

3.296E-22

2.544E-09


* Loci tested for faecal DNA samples

$ Loci used for individual identification

Deviation from Hardy-Weinberg equilibrium

Mondol et al. BMC Genetics 2009 10:79   doi:10.1186/1471-2156-10-79

Open Data