Table 6 |
|||||
|
Restriction enzymes |
|||||
|
SNP |
Enzyme |
Specificity* 5' → 3' |
Primer pair |
Units per reaction |
Incubation time |
|
|
|||||
|
ss161109995 |
Bsp143II |
PuGCGCPy |
3 |
0.5 |
14 h |
|
ss161109997 |
Bsh1236I |
CGCG |
5 |
0.5 |
14 h |
|
ss161109998 |
Eco72I |
CACGTG |
5 |
5 |
3 h |
|
ss161110009 |
Esp3I |
CGTCTC |
12 |
0.5 |
14 h |
|
ss161110010 |
EcoO109I |
PuGGNCCPy |
15 |
0.5 |
14 h |
|
|
|||||
|
*polymorphic nucleotide marked in bold |
|||||
|
Meidtner et al. BMC Genetics 2009 10:76 doi:10.1186/1471-2156-10-76 |
|||||
