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Open Access Highly Accessed Research article

Microsatellite based genetic diversity and population structure of the endangered Spanish Guadarrama goat breed

Magdalena Serrano1*, Jorge H Calvo2, Marta Martínez3, Ane Marcos-Carcavilla1, Javier Cuevas3, Carmen González1, Juan J Jurado1 and Paloma Díez de Tejada4

Author Affiliations

1 Departamento de Mejora Genética Animal, INIA. 28080 Madrid, Spain

2 Unidad de Tecnología en Producción Animal. CITA-Gobierno de Aragón. 727 50080 Zaragoza, Spain

3 CENSYRA. IMIDRA, Consejería de Economía y Consumo, Comunidad de Madrid, Ctra. de Guadalix de la Sierra, Km, 2. 28770 Colmenar Viejo, Madrid, Spain

4 Asociación de Criadores de Ganado Caprino de la Raza de Guadarrama, Ctra. de Guadalix de la Sierra, Km. 2. 28770 Colmenar Viejo, Madrid, Spain

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BMC Genetics 2009, 10:61  doi:10.1186/1471-2156-10-61

Published: 29 September 2009

Abstract

Background

Assessing genetic biodiversity and population structure of minor breeds through the information provided by neutral molecular markers, allows determination of their extinction risk and to design strategies for their management and conservation. Analysis of microsatellite loci is known to be highly informative in the reconstruction of the historical processes underlying the evolution and differentiation of animal populations. Guadarrama goat is a threatened Spanish breed which actual census (2008) consists of 3057 females and 203 males distributed in 22 populations more or less isolated. The aim of this work is to study the genetic status of this breed through the analysis of molecular data from 10 microsatellites typed in historic and actual live animals.

Results

The mean expected heterozygosity across loci within populations ranged from 0.62 to 0.77. Genetic differentiation measures were moderate, with a mean FST of 0.074, GST of 0.081 and RST of 0.085. Percentages of variation among and within populations were 7.5 and 92.5, respectively. Bayesian clustering analyses pointed out a population subdivision in 16 clusters, however, no correlation between geographical distances and genetic differences was found. Management factors such as the limited exchange of animals between farmers (estimated gene flow Nm = 3.08) mostly due to sanitary and social constraints could be the major causes affecting Guadarrama goat population subdivision.

Conclusion

Genetic diversity measures revealed a good status of biodiversity in the Guadarrama goat breed. Since diseases are the first cause affecting the census in this breed, population subdivision would be an advantage for its conservation. However, to maintain private alleles present at low frequencies in such small populations minimizing the inbreeding rate, it would necessitate some mating designs of animals carrying such alleles among populations. The systematic use of molecular markers will facilitate the comprehensive management of these populations, which in combination with the actual breeding program to increase milk yield, will constitute a good strategy to preserve the breed.