Table 1

DNA Recovery from Two 3 mm Guthrie Card Punches

    sample ID

    Yield (ng)

    Conc (ng/uL)

    Specimen Name

    Collection Date


1

261.7

26

530011

1995

2

203.37

20

529979

1995

3

162.33

16

529969

2003

4

312.9

31

529943

2003

5

380.43

38

529844

1994

6

212.16

21

529895

1994

7

319.9

32

520908

2001

8

188.41

19

529809

2001

    9

    246.88

    65

    MOD881

    1993

    10

    244.55

    65

    MOD892

    1993

11

225.47

23

529874

2003

12

131.31

13

529810

2003

    13

    391.96

    50

    MOD919

    1998

    14

    367.67

    48

    MOD937

    1998

15

161.23

16

529852

2000

16

264.59

26

529906

2000

    17

    255.67

    57

    MOD920

    1995

    18

    226.43

    65

    MOD975

    1995

    19

    268.02

    69

    MOD986

    1995

    20

    234.94

    53

    MOD001

    1995

21

350.78

35

530127

1995

22

339.53

34

530128

1991

23

224.1

22

529236

1992

24

274.61

27

529237

1997

overall average

<260 ng>

<26 ng/uL>

<1997>

    Microarray samples

<280 ng>

<59 ng/uL>

<1995>


DNA concentration was measured via PicoGreen analysis. Samples in red refer to those specimens used for microarray analysis, based on the requirement that their DNA concentration exceed 50 ng/uL to accommodate Illumina hybridization chemistry. Notice that the average DNA yield (in nanograms) for the 8 specimens used for microarray analysis and their average age did not deviate significantly from the average of all 24. However, due to volume variability in the final concentrated DNA sample, the mass concentration of the 8 specimens used for microarray analysis (59 ng/uL) was approximately twice that of the average over the entire set of twentyfour (26 ng/uL).

Hardin et al. BMC Genetics 2009 10:38   doi:10.1186/1471-2156-10-38

Open Data