Table 2

Multiplex Single Base Extension results for the HB50 bone extracts of the Iceman's DNA using the K1 multiplex.

Sample


rCRS

MP

T

T

T

C

C

T

G

C

T

A

T


HB50

K1

    C 16224C

    C 16311C

    C 16362C

C497T

C498 elC

C1189C

G5913A

T8137T

C11299C

G12308G

C12705C


For the sake of consistency with Table 1, alleles read from the forward DNA strand, relative to the revised Cambridge Reference Sequence (rCRS) [23,24], unlike Figures 2 and 3 that show the state according to the actual strand targeted by the SBE assay. These results are identical to the cloned singleplex results shown in Table 1, demonstrating consistent genotyping of the Iceman by different methods. As all these results were obtained in a single multiplex reaction, credibility is given to those reactions returning the rCRS. Such results in a singleplex scenario could be explained by contamination of an individual assay by any non hg K mtDNA source.

Endicott et al. BMC Genetics 2009 10:29   doi:10.1186/1471-2156-10-29

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