PCR results for the three extracts of the Iceman's DNA (HB49, HB50, BPE1). The results place the Iceman's mtDNA into a new (undefined) phylogenetic position (K1*) whilst also reporting the transitions at nps 8137 and 16362. Human mtDNA mutations are scored relative to the revised Cambridge Reference Sequence (rCRS) [23,24], so the Tyrolean Iceman's mtDNA is 'ancestral' at the three positions (C497T, G5913A, 498delC) that define K1a, K1b, and K1c. The sites tested in, 1 , 2  and 3this study, are shown to compare the phylogenetic discrimination achieved. Critically, the 16362C polymorphism was not reported in the original study  and not tested for in . A recent study  explored the entire mtDNA genome of the Iceman by singleplex PCR and has reported all of the polymorphisms listed in Table 1.
Endicott et al. BMC Genetics 2009 10:29 doi:10.1186/1471-2156-10-29