Research article
Development of an integrative database with 499 novel microsatellite markers for Macaca fascicularis
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* Corresponding author: Keiji Terao terao@nibio.go.jp
1 Tsukuba Primate Research Center, National Institute of Biomedical Innovation, 1-1 Hachimandai, Tsukuba, Ibaraki 305-0843, Japan
2 Department of Biomedical Resources, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan
3 Department of Research and Development, Central Blood Institute, Japanese Red Cross Society, 2-1-67 Tatsumi, Koto-ku, Tokyo 135-8521, Japan
BMC Genetics 2009, 10:24 doi:10.1186/1471-2156-10-24
Published: 5 June 2009Additional files
Additional file 1:
List of 671 primer pairs for microsatellite candidate loci. Polymorphism was determined with ten unrelated macaques. Chr, Chromosome; Y, amplification; N, no amplification; P, polymorphic; M, monomorphic. These data are also available at our website http://genebank.nibio.go.jp/cgi-bin/qfbase/macMMarker.cgi webcite, and these microsatellite markers are mapped to the rhesus macaque genome database http://genebank.nibio.go.jp/cgi-bin/gbrowse/rheMac2/ webcite. MFA0028-0061 markers are reported as NCBI/UniSTS id: markers and mapping data are 72106, 147912, 8379, 63879, 13743, 19265, 181717, 37796, 74733, 256705, 75673, 10546, 264996, 153124, 73737, 65585, 148897, 9343, 22659, 42001, 46786, 41570, 78383, 13963, 147924, 83050, 46652, 148707, 11528, 89846, 30829, 94606, 94603, and 76519, respectively.
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Additional file 2:
Allele data of 453 polymorphic markers. We checked the mode of inheritance of 453 microsatellite markers in four families (13 males and 14 females) from Malaysian cynomolgus macaques. Of these, 412 markers showed no contradictions concerning Mendelian inheritance.
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Additional file 3:
Chromosomal localization of cynomolgus macaque BAC clones. Chromosome numbers are (A): 11, 12, (B): 17, 13, (C): 7, 15, and (D): 19, 19, macaques (cynomolgus and rhesus) and human, respectively. Green spots indicate hybridization signals of BAC DNA. Background staining (red) is propidium iodide (PI).
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