Table 2

Fluorescence and absorbance properties of amphioxus GFPs representative of five clades.

GFP

Abs.

Max

(nm)

FWHM (nm)

Ex.

Max (nm)

FWHM (nm)

Em. Max (nm)

FWHM (nm)

Extinction Coefficient (M-1 cm-1) at 470 nm

Extinction Coefficient (M-1 cm-1) at 500 nm

Relative Fluorescence Intensity


GFPa1

497

45

500

38

516

39

48,100

120,900

100%

GFPe1

492

51

n.d.

n.d.

524

56

56,000

130,700

0.31%

GFPf1

504

60

n.d.

n.d.

n.f.

n.a.

13,400

25,500

n.a.

GFPd2

470

68

n.d.

n.d.

495

57

60,600

6,100

0.35%

GFPc1

493

55

n.d.

n.d.

521

56

63,900

98,800

0.33%


Excitation maximum was determined for emission set at 516 nm. Emission maximum was determined upon excitation at 500 nm for GFPa1 and at 465 nm for GFPe1, GFPf1, GFPd2, and GFPc1. Relative fluorescence intensity values are shown relative to the most fluorescent GFP, GFPa1. Abbrev.:Abs., absorbance, Ex., Excitation, Em., Emission, FWHM; full width half max, n.f., non-fluorescent; n.a., not applicable.

Bomati et al. BMC Evolutionary Biology 2009 9:77   doi:10.1186/1471-2148-9-77

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