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Open Access Research article

Molecular evolution of UCP1 and the evolutionary history of mammalian non-shivering thermogenesis

David A Hughes1*, Martin Jastroch2, Mark Stoneking1 and Martin Klingenspor3

Author Affiliations

1 Max-Planck-Institute for Evolutionary Anthropology, Department of Evolutionary Genetics, Leipzig, Germany

2 Philipps-Universität Marburg, Faculty of Biology, Department of Animal Physiology, Marburg, Germany

3 Molecular Nutritional Medicine, Technische Universität München, Else Kröner-Fresenius Center, Freising-Weihenstephan, Germany

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BMC Evolutionary Biology 2009, 9:4  doi:10.1186/1471-2148-9-4

Published: 7 January 2009

Abstract

Background

Uncoupling protein 1 (UCP1) is a mitochondrial anion carrier, expressed in brown adipose tissue (BAT) of Eutherians. UCP1 is responsible for uncoupling mitochondrial proton transport from the production of ATP, thereby dissipating heat; it is essential for non-shivering thermogenesis (NST) in mammalian BAT. UCP1 orthologs have been identified in non-Eutherian mammals, fish and amphibians. Yet, UCP1 has a unique function in Eutherians in that it is necessary in the production of heat (NST). As such, this study aims to determine the evolutionary mode of UCP1 in Eutherians, where there is clear evidence of UCP1-dependent NST in BAT.

Results

Models of adaptive evolution through phylogenetic analysis of amino acid sequences by maximum likelihood were implemented to determine the mode of UCP1 protein evolution in Eutherians. An increase in the rate of amino acid substitutions on the branch leading to Eutherians is observed, but is best explained by relaxed constraints, not positive selection. Further, evidence for branch and site heterogeneity in selection pressures, as well as divergent selection pressures between UCP1 and its paralogs (UCP2-3) is observed.

Conclusion

We propose that the unique thermogenic function of UCP1 in Eutherians may be best explained by neutral processes. Along with other evidence, this suggests that the primary biochemical properties of UCP1 may not differ between Eutherians and non-Eutherians.