Open Access Research article

Gain and loss of elongation factor genes in green algae

Ellen Cocquyt1*, Heroen Verbruggen1, Frederik Leliaert1, Frederick W Zechman2, Koen Sabbe3 and Olivier De Clerck1

Author Affiliations

1 Phycology Research Group and Center for Molecular Phylogenetics and Evolution, Ghent University, Krijgslaan 281 S8, 9000 Ghent, Belgium

2 Department of Biology, California State University, Fresno, 2555 East San Ramon Avenue, Fresno, California 93740, USA

3 Protistology and Aquatic Ecology Research Group, Ghent University, Krijgslaan 281 S8, 9000 Ghent, Belgium

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BMC Evolutionary Biology 2009, 9:39  doi:10.1186/1471-2148-9-39

Published: 12 February 2009

Additional files

Additional file 1:

Figure S1. Complete 72-taxon reference tree of SSU rDNA, rbcL and atpB.

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Additional file 2:

Figure S2. Complete phylogeny inferred from amino acid sequences of EFL and related subfamilies of the GTPase translation factor superfamily.

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Additional file 3:

Figure S3. Hypotheses about ancestral presence-absence patterns of elongation factor genes.

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Additional file 4:

Figure S4. Hypotheses about ancestral presence-absence patterns of elongation factor genes, assuming that EF-1α is not acquired by lateral gene transfer.

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Additional file 5:

Table S1. Algal strain information.

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Additional file 6:

Table S2. Genbank accession numbers for nucleotide sequences of atpB, rbcL, SSU rDNA, EF-1α and EFL, newly generated sequences are in boldface.

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Additional file 7:

Alignments. Nexus files of EF-1α, EFL, Gblocks stripped EFL and SSU-rbcL-atpB alignments.

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Additional file 8:

Additional information. Additional information on model selection procedure, and SSU rDNA alignment and partitioning strategy.

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