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Open Access Research article

High adsorption rate is detrimental to bacteriophage fitness in a biofilm-like environment

Romain Gallet12*, Yongping Shao13 and Ing-Nang Wang1

Author Affiliations

1 Department of Biological Sciences, University at Albany, Albany, NY 12222, USA

2 Current address : CEFE - UMR 5175, 1919 route de Mende, F-34293 Montpellier cedex 5, France

3 Current address : Thomas Jefferson University, Kimmel Cancer Center, Department of Cancer Biology, 233 S. 10th Street, BLSB Room 522, Philadelphia, PA 19107, USA

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BMC Evolutionary Biology 2009, 9:241  doi:10.1186/1471-2148-9-241

Published: 5 October 2009

Abstract

Background

Bacterial biofilm is ubiquitous in nature. However, it is not clear how this crowded habitat would impact the evolution of bacteriophage (phage) life history traits. In this study, we constructed isogenic λ phage strains that only differed in their adsorption rates, because of the presence/absence of extra side tail fibers or improved tail fiber J, and maker states. The high cell density and viscosity of the biofilm environment was approximated by the standard double-layer agar plate. The phage infection cycle in the biofilm environment was decomposed into three stages: settlement on to the biofilm surface, production of phage progeny inside the biofilm, and emigration of phage progeny out of the current focus of infection.

Results

We found that in all cases high adsorption rate is beneficial for phage settlement, but detrimental to phage production (in terms of plaque size and productivity) and emigration out of the current plaque. Overall, the advantage of high adsorption accrued during settlement is more than offset by the disadvantages experienced during the production and emigration stages. The advantage of low adsorption rate was further demonstrated by the rapid emergence of low-adsorption mutant from a high-adsorption phage strain with the side tail fibers. DNA sequencing showed that 19 out of the 21 independent mutant clones have mutations in the stf gene, with the majority of them being single-nucleotide insertion/deletion mutations occurring in regions with homonucleotide runs.

Conclusion

We conclude that high mutation rate of the stf gene would ensure the existence of side tail fiber polymorphism, thus contributing to rapid adaptation of the phage population between diametrically different habitats of benthic biofilm and planktonic liquid culture. Such adaptability would also help to explain the maintenance of the stf gene in phage λ's genome.