Table 3 |
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|
Oligonucleotides |
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|
name |
sequence (5' to 3')* |
target (peptide sequence)** |
|
|
||
|
P-486 |
GAGCGGATAACAATTTCACACAGG |
pUC/M13 rv |
|
P-517 |
CCTACCCCATGAGAGGCCGTTGGA |
A. pernix, fw |
|
P-518 |
GGCCTATGGAGCTGCATCACGCA |
A. pernix, rv |
|
P-583 |
GTTTTCCCAGTCACGACGTTGTA |
pUC/M13 fw |
|
P-1516 |
gagcggataacaatttcacacaggaVKGGKGGYYTYTGRTADAT |
cbf5, rv (IYQ(K/R)PP(L/V)) |
|
P-1607 |
gttttcccagtcacgacgttgtaGGKCCKACKTCKCAYGARGT |
cbf5, fw (GPTSHEV) |
|
P-1608 |
gttttcccagtcacgacgttgtaGGKCCKACKAGYCAYGARGT |
cbf5, fw (GPTSHEV) |
|
P-1609 |
gagcggataacaatttcacacaggARYTCKCCYTTNAGNGT |
cbf5, rv (TLKGEL) |
|
P-1610 |
gagcggataacaatttcacacaggARYTCKCCYTTYAANGT |
cbf5, rv (TLKGEL) |
|
P-1835 |
gttttcccagtcacgacgttgtaGGKCCKACNAGYCAYGA |
cbf5 fw (GPTSHE) |
|
P-1838 |
gagcggataacaatttcacacaggTKGGRTCNAGNGTNCC |
cbf5 rv (TTLDP(K/N/R)) |
|
P-1856 |
GGTTGTAGCGTGGCTTAGGAAGCT |
T. pendens, fw |
|
P-1857 |
GCTCCTAGGGATAGAGAGAATAGC |
T. pendens, fw |
|
P-1860 |
TCGAACCTCCCTCTTCACAGCAGA |
Thermofilum, rv |
|
P-1862 |
CAGCTTCGCACCAGACATGGAGGA |
Thermofilum, rv |
|
P-1911 |
gttttcccagtcacgacgttgtaGGKCCKAGYAGYCAYGA |
cbf5, fw (GPSSHE) |
|
|
||
|
*; sequencing primer binding site is shown in lower case. **; fw; forward, rv; reverse K = G or T; R = G or A; Y = T or C; N = T, C, G or A; D = T, G, or A; V = C, G or A; S = C or G. |
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|
Yokobori et al. BMC Evolutionary Biology 2009 9:198 doi:10.1186/1471-2148-9-198 |
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