Research article
The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
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* Corresponding author: Claudia MA Carareto carareto@ibilce.unesp.br
- † Equal contributors
1 UNESP – São Paulo State University, Laboratory of Molecular Evolution, Department of Biology, 15054-000 São José do Rio Preto, São Paulo, Brazil
2 Université de Lyon, F-69000, Lyon; Université Lyon 1; CNRS, UMR5558, Laboratoire de Biométrie et Biologie Evolutive, F-69622, Villeurbanne, France
BMC Evolutionary Biology 2009, 9:174 doi:10.1186/1471-2148-9-174
Published: 22 July 2009Additional files
Additional file 1:
Sequences of the Helena reference copies. Helena sequences used to determine the structure of the element, for each species.
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Additional file 2:
Helena copies in the Drosophila sechellia sequenced genome. The data provided is a list of D. sechellia copies.
Format: DOC Size: 246KB Download file
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Additional file 3:
Helena copies in the Drosophila erecta sequenced genome. The data provided is a list of D. erecta copies.
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Additional file 4:
Helena copies in the Drosophila ananassae sequenced genome. The data provided is a list of D. ananassae copies.
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Additional file 5:
Helena copies in the Drosophila yakuba sequenced genome. The data provided is a list of D. yakuba copies.
Format: DOC Size: 52KB Download file
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Additional file 6:
Helena copies in the Drosophila virilis sequenced genome. The data provided is a list of D. virilis copies.
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Additional file 7:
Helena copies in the Drosophila mojavensis sequenced genome. The data provided is a list of D. mojavensis.
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Additional file 8:
Schematic representation of Helena. Schematic representation of Helena copies in D. sechellia, D. yakuba, D. erecta, D. ananassae, D. mojavensis and D. virilis. The sequences represented have at least 90% identity and 50% of the length of the reference copy, and with e-values of less than 10e-10. Spaces = indels. The first schematic representation is the reference copy in each species. White = gag. Gray = RTase.
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Additional file 9:
Species and strains, geographic origin and year of collection. The data provided is a list of D. simulans and D. mojavensis used in this study for analysis of Helena activity in natural populations.
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Additional file 10:
Mean CT of the rp49 control gene and Helena. Ct comparison between rp49 (reference) and Helena real time PCR assays of D. simulans and D. mojavensis. Gray = mean CT of D. simulans. Black = mean CT of D. mojavensis. Std = standard deviation.
Format: TIFF Size: 381KB Download file