Figure 3.

Kinetic performance across a temperature gradient of 3 Idh-1 alleles in the cricket Allonemobius socius. Enzyme efficiency (A) was measured as the increase in reaction velocity with increasing substrate concentration (see Methods). K_m (B) and V_max (C) were calculated using standard Michaelis-Menten methods. Means ± SE are given (n = 3 individuals per genotype). Individual ANOVA's were used to test for differences between genotypes at each temperature and significant differences indicated (*P < 0.1, **P < 0.05, ***P < 0.01).