|
Specific primers designed from SNPs in the dimeric α-amylase inhibitor genes |
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| Primer |
Sequences* |
Fragment size |
Temperature |
Cycle |
|
|
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| W19G |
ATGCTCGTGGCGACACTCG |
426 |
64 |
32 |
| W24A |
TCGTGGCGACACCCRTACCA |
422 |
63 |
35 |
| W35A |
ACCCATAGCAGCCCAGTACAA |
412 |
62 |
35 |
| W46A |
CGAGTACGACGCATGGATCA |
400 |
65 |
35 |
| W47AT |
AGTACGACGCATGGAGTAT |
398 |
57 |
35 |
| W125G |
CTGTCGTCCATTGCTTAG |
319 |
62.5 |
35 |
| W127G |
CTGTCGTCCATTGCTGAGGG |
319 |
62.5 |
35 |
| W190C |
CTGCTGCCAGCAGCTCGGCC |
256 |
57 |
35 |
| W195T |
TGCCAGCAGCTCGCCGACATT |
252 |
51 |
35 |
| W207T |
ACATCAGCGAGTGGAGT |
236 |
60.5 |
30 |
| W259A |
CATGTATAAGGAGCATGTCA |
187 |
61 |
35 |
| W263C |
TATAAGGAGCATGGCGTCTC |
183 |
62 |
30 |
| W263A |
TATAAGGAGCATGGCGTCTA |
183 |
65 |
35 |
| W276A |
GCGTGTCGGAGGGACAGTCA |
170 |
60 |
35 |
| W288CG |
GACAGGCRGGGACAGGAACG |
158 |
66 |
30 |
| W288AG |
GACAGGCGGGGACAGGAAAG |
158 |
63.5 |
30 |
|
*SNP positions were on the 3' end of the primers and are identified in bold letters. Extra mismatched nucleotides were also incorporated in the primers (underlined). | ||||
Wang et al. BMC Evolutionary Biology 2008 8:91 doi:10.1186/1471-2148-8-91 |
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