|
Primers and PCR conditions. |
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| Gene |
Primers |
Sequence (5'-3') |
Tm (°C) |
Size (pb) |
Reference |
|
|
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| RAG1 |
RAG1 (a, s) |
AGCTGTAGTCAGTAYCACAARATG |
|||
| RAG5 (s) |
TRGAGTCACACAGACTGCAGA |
58* |
1512 |
[67]. |
|
| RAG9 (a, s) |
GTGTAGAGCCAGTGRTGYTT |
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| Cytochrome oxidase I (COI) |
FISHF1 (a, s) |
TCAACCAACCACAAAGACATTGGCAC |
|||
| FISHR1 (a) |
TAGACTTCTGGGTGGCCAAAGAATCA |
54 |
655 |
[83] |
|
| Cytochrome b (Cytb) |
Glu- F (a, s) |
GAAGAACCACCGTTGTTATTCAA |
|||
| Thr- R (a, s) |
ACCTCCRATCTYCGGATTACA |
48 |
[84] |
||
| CbCHR (a, s) |
TTARTCCGGCTGGGWTNTTTG |
48 |
1140 |
This study |
|
| 16 S |
16SAR (a, s) |
CGCCTGTTTATCAAAAACAT |
|||
| 16SBR (a) |
CCGGTCTGAACTCAGATCACGT |
46 |
552 |
[85]. |
|
|
Amplification (a) and sequencing (s) primers used for the RAG1, 16S, COI, and Cytb genes. *Touchdown PCR was performed for the first 10 cycles: from 58°C to 53°C (-0.5°C each cycle), followed by 25 cycles at 53°C. | |||||
Ornelas-García et al. BMC Evolutionary Biology 2008 8:340 doi:10.1186/1471-2148-8-340 |
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