Additional file 1:

Individuals used in phylogenetic analyses. Gene accession number and locality of alleles and individuals included in the phylogenetic analysis.

Format: DOC Size: 204KB Download file

This file can be viewed with: Microsoft Word Viewer

Additional file 2:

Forewing size between H. cydno and H. melpomene. Wild individuals from Florencia and museum specimens of H. cydno and H. melpomene were measured for forewing area. Wings were scanned next to a ruler and processed in tpsUtil and tpsDig 9 image software to measure area. Tests for normal distribution were carried out for each species and a one-way Analysis of Variance (ANOVA) by species and sex was performed in SPSS to test for differences in size. Tukey's post-hoc was used to compare median values between pairwise population sets. H. m. malleti did not differ in wing area from other H. melpomene races (ANOVA p > 0,05). H. cydno cognate wing area is similar to H. cydno races (ANOVA p > 0,05).

Format: TIFF Size: 2MB Download file

Additional file 3:

Larvae colour dispersion index b' based on intensity and brightness of the head capsule. H. melpomene has a light dust cephalic tone with dark and broad bands, while H. cydno has a dark yellow-orange cephalic tone and light narrow bands. b' = b/(r+g+b) based on RGB filters using the procedure as in Endler et al. 1990. H. cydno races are: H. c. cordula, H. c. cydnides and H. c. zelinde and H. melpomene races are H. m. mocoa and H. m. vulcanus. Analysis of variance shows high differences between melpomene and cydno groups (H. melpomene and H. m. malleti vs. H. cydno and H. cydno cognate; p < 0,0001).

Format: TIFF Size: 2.2MB Download file

Additional file 4:

Best cluster assignment. Magnitude of ΔK as a function of K (mean ± SD over 5 replicates), calculated using the procedure of Evanno et al. (2005). a) ΔK for Structure (Ln for K = 2:-4691,42) and b) ΔK for BAPS 4 (Ln for K = 2:-4987,95).

Format: TIFF Size: 499KB Download file