Table 1

Primers used in this study.

Primer

Sequence

P1

5'-CCTCTTCTCTTGTTGccATGGAG-3'

P2

5'-CAGTACAGCAAATTAccaTGGGTTTG-3'

P3

5'-TTTCCCTGTCTTATTGCcATGGAA-3'

P4

5'-CCAGTACAGCATATTAccaTGGGTTTA-3'

P5

5'-CTGCTTCAAACtgtACaTAATTTGC-3'

P6

5'-GAAAGTAAAATgcGGccGCTCATCC-3'

P7

5'-TTGTTTCTGAAACTTGGTTACAG-3'

P8

5'-TTACTTCTTTTGCTTCTTCCCTGGCTT-3'

P9

5'-AATGG(A/C)AC(C/T)G(A/G)(A/T)ATGGTCAAG-3'

P10

5'-TTAGAAGCA(C/T)TTC(A/C)TGTG(C/G)AC-3'

Underline: restriction sites for BsrGI, NcoI and NotI.

Lowercase letters: deviations from the original OsRpl6-1 sequence to enable introduction of restriction sites.

The locations of primers P1-P8 are shown in Figure 1A.

Primers P9 and P10 were designed from rice Actin sequences [39] as described previously [40] with slight modifications.

Kubo et al. BMC Evolutionary Biology 2008 8:314   doi:10.1186/1471-2148-8-314