Table 4

Oligonucleotide primers used in this study

Gene

Primer Name

Primer Sequence

Base Position

Reference

COI

LCO1490

F

GGT CAA CAA ATC ATA AAG ATA TTG G

1501 (38)

[57]

DanausCOI-F3

F

GTT TGA GCA GTA GGT ATY ACA GC

2029 (566)

this study

ButterCOI-R2

R

GTA ATT GCY CCA GCT AAA ACW GG

2074 (611)

this study

TN2126

F

TTG AYC CTG CAG GTG GWG GAG

2133 (670)

R. Eastwood, unpublished

Nancy

R

CCC GGT AAA ATT AAA ATA TAA ACT TC

2203 (740)

[58]

ButterCOI-F4

F

GAA TAA TTT ATG CAA TAW TAG CWA TTG G

2296 (833)

this study

ButterCOI-R3

R

CCA ACT GTA AAT ATA TGA TGR GCT C

2341 (878)

this study

ButterCOI-R4

R

GAT AAW ACA TAA TGR AAA TGT GCT AC

2599 (1136)

this study

Hobbes

R

AAA TGT TGN GGR AAA ATG TTA

2743 (1280)

[59]

cytB

REVCB2H

F

TGA GGA CAA ATA TCA TTT TGA GGW

10964 (438)

[60]

REVCBJ

R

ACT GGT CGA GCT CCA ATT CAT GT

11566 (1040)

[60]

Wolbachia wsp

81F

F

TGG TCC AAT AAG TGA TGA AGA AAC

(81)

[61]

691R

R

AAA AAT TAA ACG CTA CTC CA

(691)

[61]

Direction of amplification is given with reference to the 5' end of the gene. Base position of the primer denotes the position of the last nucleotide on the 3' end of the primer from the 5' end of the Coreana raphaelis (Lepidoptera: Lycaenidae) mitochondrial genome and (individual gene sequence) [28]. F = forward primer; R = reverse primer

Lohman et al. BMC Evolutionary Biology 2008 8:301   doi:10.1186/1471-2148-8-301