Additional file 1:

Sequence alignment of the ^3'IGS-ETS sequences in Bacillus. Sequence alignment and annotation of the ^3'IGS-ETS sequences in Bacillus. Species acronyms as in Table 1. Putative functional sequences are marked in bold. The stem and loop structure at the boundary between ETS and 18S gene is reported above the sequence at the appropriate position of the alignment.

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Additional file 2:

Sequence alignment of the Bag530 sequences in Bacillus. Sequence alignment of the Bag530 sequences in Bacillus. Species acronyms as in Table 1. The "C" followed by a number indicates the number of clone sequenced.

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Additional file 3:

Putative promoter sequences comparisons. Putative promoter sequence of B. atticus and B. grandii (Ba-g). Promoter sequences of Triops cancriformis (Tca), Daphnia pulex (Dpu) and Artemia franciscana (Afr) were also reported for comparison. The first nucleotide of the promoter sequence is indicated by (>) and seven bp of the upstream sequence are reported; (g) and (s) indicate gene and spacer promoters respectively. Consensus sequence of regions surrounding rDNA tsp of several arthropods is reported, as described by Crease (1993).

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Additional file 4:

Secondary structure of the ETS-18S junction. A) Secondary structure of the ETS-18S junction. Calculated free energy is reported below the structure. B) Conserved rRNA helices (H9, H17 and H9') flanking 3'-end ETS are within dashed vertical line

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