Result of sequence similarity search for AMEL in the target region of the chicken genome. This region is delimited by two flanking genes, ARHGAP6 (exon 2) and MID1. This region (200 kb) was extracted, and a similarity search was performed using crocodile AMEL exon 2, then exons 3 and 5 and the beginning of exon 6 (Figure 5). We used UniDPlot software (Girondot and Sire, unpublished), an extension of the dot-plot method, in which the maximum similarity index between both sequences is shown on the axis of the largest sequence. Significant identity was tested by calculating the distribution under H0 limits obtained by random sampling of sequences. Top: Candidate region of chicken DNA showing the hits. Bottom: Detail of the chicken AMEL gene region found 38 kb from the 5' region of ARHGAP6 exon 2.
Sire et al. BMC Evolutionary Biology 2008 8:246 doi:10.1186/1471-2148-8-246