Table 3

Standard curve log fit values for quantitative PCR.

(Plasmid) Standard Curve

Linear Equation

Efficiency (%)

RSq


A180

Y = -3.315(logX) + 37.05

100.3

0.999

S180

Y = -2.992(logX) + 35.77

115.9

0.996

S180r

Y = -3.670(logX) + 38.96

87.3

0.985

P180

Y = -3.319(logX) + 36.68

100.1

0.999


The linear equation, efficiency and r-squared (RSq) values for the qPCR standard curve are shown. Plasmid copy numbers used as standards were determined by calculating the number of moles in each sample multiplied by Avogadro's number. A standard curve of Ct (dR) was used to calculate original copy number of mRNA transcripts in the diluted cDNA samples.

Ward et al. BMC Evolutionary Biology 2008 8:210   doi:10.1186/1471-2148-8-210

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