Table 3 

Standard curve log fit values for quantitative PCR. 

(Plasmid) Standard Curve 
Linear Equation 
Efficiency (%) 
RSq 


A180 
Y = 3.315(logX) + 37.05 
100.3 
0.999 
S180 
Y = 2.992(logX) + 35.77 
115.9 
0.996 
S180r 
Y = 3.670(logX) + 38.96 
87.3 
0.985 
P180 
Y = 3.319(logX) + 36.68 
100.1 
0.999 


The linear equation, efficiency and rsquared (RSq) values for the qPCR standard curve are shown. Plasmid copy numbers used as standards were determined by calculating the number of moles in each sample multiplied by Avogadro's number. A standard curve of Ct (dR) was used to calculate original copy number of mRNA transcripts in the diluted cDNA samples. 

Ward et al. BMC Evolutionary Biology 2008 8:210 doi:10.1186/147121488210 