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Open Access Highly Accessed Research article

Genetic evidence from Indian red jungle fowl corroborates multiple domestication of modern day chicken

Sriramana Kanginakudru13, Muralidhar Metta1, RD Jakati2 and J Nagaraju1*

Author Affiliations

1 Laboratory of Molecular Genetics, Centre for DNA Fingerprinting and Diagnostics, ECIL-Road, Nacaharam, Hyderabad, 500076, India

2 Van Bhavan, Plot No 6-18, Sector 6, Panchakula, Haryana, India

3 Department of Neurobiology, University of Massachusetts Medical School, Worcester, MA 01605, USA

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BMC Evolutionary Biology 2008, 8:174  doi:10.1186/1471-2148-8-174

Published: 10 June 2008

Abstract

Background

Domestication of chicken is believed to have occurred in Southeast Asia, especially in Indus valley. However, non-inclusion of Indian red jungle fowl (RJF), Gallus gallus murghi in previous studies has left a big gap in understanding the relationship of this major group of birds. In the present study, we addressed this issue by analyzing 76 Indian birds that included 56 G. g. murghi (RJF), 16 G. g. domesticus (domestic chicken) and 4 G. sonneratii (Grey JF) using both microsatellite markers and mitochondrial D-loop sequences. We also compared the D-loop sequences of Indian birds with those of 779 birds obtained from GenBank.

Results

Microsatellite marker analyses of Indian birds indicated an average FST of 0.126 within G. g. murghi, and 0.154 within G. g. domesticus while it was more than 0.2 between the two groups. The microsatellite-based phylogenetic trees showed a clear separation of G. g. domesticus from G. g. murghi, and G. sonneratii. Mitochondrial DNA based mismatch distribution analyses showed a lower Harpending's raggedness index in both G. g. murghi (0.001515) and in Indian G. g. domesticus (0.0149) birds indicating population expansion. When meta analysis of global populations of 855 birds was carried out using median joining haplotype network, 43 Indian birds of G. g. domesticus (19 haplotypes) were distributed throughout the network sharing haplotypes with the RJFs of different origins.

Conclusion

Our results suggest that the domestication of chicken has occurred independently in different locations of Asia including India. We found evidence for domestication of Indian birds from G. g. spadiceus and G. g. gallus as well as from G. g. murghi, corroborating multiple domestication of Indian and other domestic chicken. In contrast to the commonly held view that RJF and domestic birds hybridize in nature, the present study shows that G. g. murghi is relatively pure. Further, the study also suggested that the chicken populations have undergone population expansion, especially in the Indus valley.