Figure 1.

GPCR sequence analysis strategy employed. The Ciona Proteome was searched for 6/7/8 'TM' segment spanning sequences and the hits were taken for comparison against GPCRDB and against customized HMMs and PSSMs of GPCR families/subfamilies using an array of similarity/pattern search tools like BLASTP, HMMPFAM and RPS-BLAST. Subsequently, a number of phylogenetic analyses were performed on the sequences identified as putative GPCRs.