Table 4

Primers used for PCR, RACE, and genome-walking to generate rpoD and tsg1 gene sequences.

Primer name

Sequence

PCR

M13 forward

5'-GTAAAACGACGGCCAG-3'

M13 reverse

5'-CAGGAAACAGCTATGAC-3'

ORAC 1104

5'-AGGCTGACGAAGCTTGTGCAA-3'

ORAC 1105

5'-TTCTCTACCTACGCAACATGGTGG-3'

RACE

ORAC 240

5'-GATACGGTGAAGGACAAGGTCTCATC-3'

ORAC 242

5'-CAGTGGCTTCTGCAAGCTCCGCGAG-3'

Genome walking

ORAC 231

5'-TACCCAAAGTCTTCTCCAGTGTAACTAGAG-3'

ORAC 230

5'-GAATTTCTTCTTGTGCCGTCAACATATCG-3'

Duplessis et al. BMC Evolutionary Biology 2007 7:70   doi:10.1186/1471-2148-7-70