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Open Access Research article

A canonical FtsZ protein in Verrucomicrobium spinosum, a member of the Bacterial phylum Verrucomicrobia that also includes tubulin-producing Prosthecobacter species

Benjamin Yee1, Feras F Lafi1, Brian Oakley2, James T Staley2 and John A Fuerst1*

Author Affiliations

1 School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia

2 Department of Microbiology, University of Washington, Seattle, WA 98195, USA

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BMC Evolutionary Biology 2007, 7:37  doi:10.1186/1471-2148-7-37

The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2148/7/37


Received:3 November 2006
Accepted:12 March 2007
Published:12 March 2007

© 2007 Yee et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

The origin and evolution of the homologous GTP-binding cytoskeletal proteins FtsZ typical of Bacteria and tubulin characteristic of eukaryotes is a major question in molecular evolutionary biology. Both FtsZ and tubulin are central to key cell biology processes – bacterial septation and cell division in the case of FtsZ and in the case of tubulins the function of microtubules necessary for mitosis and other key cytoskeleton-dependent processes in eukaryotes. The origin of tubulin in particular is of significance to models for eukaryote origins. Most members of domain Bacteria possess FtsZ, but bacteria in genus Prosthecobacter of the phylum Verrucomicrobia form a key exception, possessing tubulin homologs BtubA and BtubB. It is therefore of interest to know whether other members of phylum Verrucomicrobia possess FtsZ or tubulin as their FtsZ-tubulin gene family representative.

Results

Verrucomicrobium spinosum, a member of Phylum Verrucomicrobia of domain Bacteria, has been found to possess a gene for a protein homologous to the cytoskeletal protein FtsZ. The deduced amino acid sequence has sequence signatures and predicted secondary structure characteristic for FtsZ rather than tubulin, but phylogenetic trees and sequence analysis indicate that it is divergent from all other known FtsZ sequences in members of domain Bacteria. The FtsZ gene of V. spinosum is located within a dcw gene cluster exhibiting gene order conservation known to contribute to the divisome in other Bacteria and comparable to these clusters in other Bacteria, suggesting a similar functional role.

Conclusion

Verrucomicrobium spinosum has been found to possess a gene for a protein homologous to the cytoskeletal protein FtsZ. The results suggest the functional as well as structural homology of the V. spinosum FtsZ to the FtsZs of other Bacteria implying its involvement in cell septum formation during division. Thus, both bacteria-like FtsZ and eukaryote-like tubulin cytoskeletal homologs occur in different species of the phylum Verrucomicrobia of domain Bacteria, a result with potential major implications for understanding evolution of tubulin-like cytoskeletal proteins and the origin of eukaryote tubulins.

Background

Both eukaryotes and prokaryotes are now known to possess cytoskeletal proteins, including tubulin components of the eukaryotic microtubules important in mitosis and the tubulin homolog FtsZ of bacteria which forms a septal Z-ring during bacterial cell division[1,2]. Most bacteria possess a cell division protein more closely related to FtsZ, the homolog of tubulin present almost universally within bacteria, than to the tubulin characteristic of eukaryotes [3-5]. Some rare bacterial species appear to lack any tubulin or FtsZ homolog at all [4]. Both FtsZ and tubulin are significant for evolutionary cell biology since FtsZ is pivotal to cell division in most bacteria and tubulin is a cytoskeletal eukaryote signature protein [6]central to models for origins of eukaryote cell organization [7,8]. Tubulin has been confirmed to be the homolog of FtsZ at the 3D structural level by crystallography [9,10]. Both proteins can polymerize into filaments, in the case of tubulin as a heterodimer of alpha and beta subunits that forms the basis in vivo of eukaryote cytoskeletal microtubules central to many processes of eukaryote cell biology, and in the case of FtsZ into protofilaments in vitro and septal rings in vivo [2].

The genus Prosthecobacter is a member of the phylogenetically divergent phylum Verrucomicrobia [11] which forms a superphylum with other phyla Planctomycetes and Chlamydia of the domain Bacteria [12], and species of this genus such as P. dejongeii, possess the proteins BtubA and BtubB more closely related to eukaryotic tubulin than to bacterial FtsZ [13]. BtubA and BtubB appear to be genuine tubulins, and can form three-dimensional bundles of up to 30 protofilaments in the presence of GTP in vitro [14]. Prosthecobacter dejongeii, however, does not apparently possess FtsZ [14]. It would be expected that other members of the phylum Verrucomicrobia would also possess such a tubulin homolog e.g. if a last common ancestor of the verrucomicrobia also possessed such a homolog. We therefore searched the draft genome of Verrucomicrobium spinosum for the presence of genes similar to cytoskeletal proteins such as FtsZ and tubulin, and revealed the presence of a gene coding for a protein with primary and secondary structural characteristics and evolutionary relationships consistent with closer relationship to genes for bacterial FtsZ than to those for eukaryotic tubulins.

Results and discussion

A putative gene (Genbank: DQ845344) which appears to code for an ortholog of bacterial FtsZ, a cytoskeletal protein involved in cell division, was amplified to confirm the sequence from examining the draft genome of Verrucomicrobium spinosum. The translated protein VerFtsZ from this putative gene is 673 amino acids long, and application of FingerPRINTScan showed that VerFtsZ contains each of the six motifs specific to bacterial FtsZ [15]. Consistent with this, application of GeneFIND for gene family identification showed a highly probable relationship to the FtsZ and FtsZ1 superfamily[16].

The core region of FtsZ in bacteria starts with a highly conserved isoleucine with only 14 changes at the position in 225 sequences from different organisms and ends with a recognizable version of the amino acid sequence LVITG. The core region of bacterial FtsZ also contains a sequence similar to the highly conserved tubulin signature motif (PROSITE motif PS00227: ([S/A/G]GGTG [S/A/T]G). VerFtsZ aligns perfectly with the conserved isoleucine of other FtsZ sequences at the start of the core region and also contains the tubulin signature – as manifested by the sequence GGGTGSG (Fig. 1). However, VerFtsZ shows a fairly divergent sequence at the end of the core region (Fig. 1), which was observed in seven sequences of Archaea FtsZ3, as well as in Mycoplasma pneumoniae and Mycoplasma genitalium FtsZ. The C-terminal region of bacteria, which interacts directly with FtsA, lacks a classical consensus sequence due to substitutions but a sequence logo has been derived from an alignment to display the pattern of protein sequences at the C-terminal [4], and there is some relative conservation of amino acids at particular positions. The C-terminal of VerFtsZ seemed to vary slightly from this sequence logo but it does share the highly conserved Proline position (position number 728 in Fig. 1) with other FtsZ sequences.

thumbnailFigure 1. Sequence alignment of VerFtsZ of Verrucomicrobium spinosum with reference amino acid sequences of FtsZ from representative Bacteria with highlight of residues based on an identity threshold of at least 50% across taxa. The first and last block of the alignment shows the N-terminal and C-terminal region respectively while the middle four blocks form the core region (the first 70 poorly alignable residues of the N-terminal region are not shown). Identities are denoted with a '*' below the amino acid position. Red arrows indicate amino acid positions at which the sequence of Verrucomicrobium spinosum differs from the FtsZ sequences of all other Bacterial phyla in the alignment. The blue arrow indicates the position of uncommon Serine within the tubulin signature motif. L.lactis, Lactococcus lactis; N.gonorrhoeae, Neisseria gonorrhoeae; B. fragilis, Bacteroides fragilis; P. albensis, Prevotella albensis; N. punctiformae, Nostoc punctiformae; G. violaceus, Gloeobacter violaceus; B. cereus, Bacillus cereus; E. coli, Escherichia coli; T. pallidum, Treponema pallidum; L. interrogans, Leptospira interrogans; T. maritima, Thermotoga maritima; A. aeolicus, Aquifex aeolicus; VerFtsZ, Verrucomicrobium spinosum.

A more detailed examination of the core region of VerFtsZ reveals significant divergence at amino acid positions otherwise conserved among other phyla. There are 8 instances (red arrows in Fig. 1) of amino acid changes to another amino acid of different physico-chemical properties from those conserved completely in the other sequences, which could imply possible structural and physiochemical significance e.g. hydrophilic to hydrophobic for the change of lysine to cysteine at position 75. The amino acid serine within the tubulin signature motif (highlighted with blue arrow at position 174) presents yet another significant difference. An examination of available database for the tubulin signature motif at PROSITE, and that compiled by Vaughan et al., revealed that a serine at this position is present only in the FtsZ of the alpha-proteobacterium Magnetospirillium magnetotacticum, plastids of some members under Viridiplantae and the Archaea Halobacterium species NRC-1, but highly conserved throughout the eukaryotic tubulins and also in BtubA and BtubB of Prosthecobacter.

Preliminary deduction of VerFtsZ protein structure from secondary structure comparisons using Cn3D database at NCBI resulted in reasonable alignment to the structural model of 1FSZ for FtsZ of Methanococcus jannaschii from Protein Data Bank (note that this species is synonymous with Methanocaldococcus jannaschii, the name used in some other databases employed for this study).

In order to investigate the relationship of VerFtsZ to FtsZ of other bacteria, we performed phylogenetic analysis using the alignment in Fig 1 which consists of a set of reference sequences of FtsZ of bacteria of different phyla. The alignment contains the functional core and C-terminal domains of FtsZ while the variable spacer region has been omitted. Phylogenetic analysis of an alignment including VerFtsZ and reference sequences of FtsZ from bacteria was performed using Treefinder [17]. In the analysis (Fig. 2), the long branch of VerFtsZ confirms its divergence from the other representatives of FtsZ among the domain Bacteria with no apparent implication of horizontal gene transfer from any existing bacterial phyla.

thumbnailFigure 2. Maximum Likelihood phylogenetic tree including VerFtsZ (red text) and reference FtsZ sequences from Bacteria. The tree was derived via TreeFinder and bootstrap confidence values are based on 1,000 replicates. Scale bar represents 0.1 substitutions per site. L.lactis, Lactococcus lactis; N.gonorrho, Neisseria gonorrhoeae; B. fragilis, Bacteroides fragilis; P. albensis, Prevotella albensis; N. punctifo, Nostoc punctiformae; G. violaceu, Gloeobacter violaceus; B. cereus, Bacillus cereus; E. coli, Escherichia coli; T. pallidum, Treponema pallidum; L. interrog, Leptospira interrogans; T. maritima, Thermotoga maritima; A. aeolicus, Aquifex aeolicus; VerFtsZ, Verrucomicrobium spinosum FtsZ.

VerFtsZ displays lower similarity to FtsZs of other Bacterial phyla than the similarity of the FtsZs of any of those phylum representatives to the FtsZs from any other Bacterial phylum. For example, when calculated using an alignment without the variable spacer region, the FtsZ of Aquifex aeolicus, widely considered a deep-branching bacterium, exhibits a similarity to VerFtsZ of only 36.8%, yet shows a similarity of at least 42% to the other representative FtsZs, and other Bacterial FtsZs often display greater similarity to any other Bacterial FtsZs than to VerFtsZ.

In other Bacteria, the ftsZ gene is known to form part of the dcw gene cluster which possesses other genes coding for proteins concerned with cell division coordinated with FtsZ in the divisome, such as FtsA, FtsI, FtsW, FtsQ and several genes concerned with cell wall synthesis such as the peptidoglycan synthesis mur genes murG, murD and murE [18]. V.spinosum contains an almost complete set of proteins of the dcw gene cluster (Fig. 3), with only MraZ and FtsL missing from the cluster. The genes for MurB, MurC and EnvA are absent from the V. spinosum dcw cluster but corresponding sequences are present elsewhere in the genome of this species. The order of the genes in the dcw cluster in V. spinosum can be seen to be similar to the gene order of the dcw clusters of bacteria in several distinct phyla (as represented by Escherichia coli, Bacillus subtilis, Thermotoga maritima and Chlamydia trachomatis in Fig. 3, members of the phyla Proteobacteria, Firmicutes, Thermotoga, and Chlamydia respectively). Despite sharing membership of a potential superphylum with Chlamydia trachomatis, the dcw cluster of V. spinosum, like E. coli and B. subtilis, possesses FtsQ, FtsA and FtsZ in that same order, while these are missing from the dcw cluster of C. trachomatis, an organism known to possess no FtsZ.

thumbnailFigure 3. Order of dcw cluster proteins in V.spinosum as compared to those of selected reference bacteria [39]. The key to one letter gene symbols are as follows : R, mraZ; M, mraW; L, ftsL; I, ftsI; E, murE; F, murF; Y, mraY; D, murD; W, ftsW; G, murG; C, murC; B, murB; d, ddlB; Q, ftsQ; A, ftsA; Z, ftsZ; V, envA(lpxC). Full species names are E.coli, Escherichia coli; B.subtilis, Bacillus subtilis; C.trachomatis, Chlamydia trachomatis; T.maritima, Thermotoga maritima; V.spinosum, Verrucomicrobium spinosum. '+' symbols represent a homologue is present at a different location within the genome, '-' symbols represent no recognizable homologue found and is only used where complete genome sequences are available, '?' symbols indicate a possible homologue. It should be noted that although genes are often shown as if contiguous, in some cases genes are separated from the next downstream e.g. in C. trachomatis, murE is separated from murF. Also, some genes are actually contiguous but a + sign intervenes only to indicate that a homolog missing in an expected position in between them is present but in another part of the genome.

The organization of the ftsZ gene of V. spinosum within a gene cluster known to be concerned with formation of a septation divisome and related cell wall synthesis in E. coli suggests that the verrucomicrobial FtsZ protein also participates in a multi-protein divisome organizing septum synthesis.

Conclusion

It is clear from the above analysis that VerFtsZ of the verrucomicrobia member Verrucomicrobium spinosum is a true ortholog of other FtsZ sequences from Bacteria, and differs markedly from the tubulin homologs BtubA and BtubB of Prosthecobacter dejongeii. BtubA and BtubB have been shown to form a similar heterodimer to αβ-tubulin and seem to be bacterial tubulins fundamentally distinct from FtsZ while sharing the features common to the FtsZ-tubulin family [19]. This difference between the V. spinosum FtsZ and the P. dejongeii tubulins is of considerable evolutionary interest, since clearly from our analysis, these two markedly different proteins occur in two species within the same phylum Verrucomicrobia of domain Bacteria. One would expect from phylogenetic considerations that members of the same monophyletic phylum level group within the Bacteria would possess orthologs of only FtsZ or tubulin but that both of these closely related but quite distinct orthologs would not be present in the same phylum. The occurrence of both FtsZ and tubulin homologs within different genera of the same phylum can be explained either by a gene loss and replacement by a lateral gene transfer event or by a close relationship of the FtsZ and tubulins found in this phylum to a last common ancestor possessing a protein with similarities to both. It has been pointed out from structural considerations that horizontal gene transfer is one explanation for the origin of the tubulin homologs BtubB and BtubA in P. dejongeii [19].

The unexpected occurrence of both FtsZ and tubulin cytoskeletal proteins in one bacterial phylum could be explained in several ways. Both proteins could have been present in the common ancestor of Verrucomicrobium and Prosthecobacter and then may have been differentially lost or have significantly diverged. Alternatively, horizontal transfer from proto-eukaryotes or eukaryotes to Prosthecobacter may account for tubulin in this genus and this is supported by genome data suggesting a largely bacterial nature for P.dejongeii genes [19].

Verrucomicrobium spinosum and Prosthecobacter dejongeii form a relatively shallow clade within the verrucomicrobia according to other genes. The 16S rRNA similarity between Verrucomicrobium spinosum and Prosthecobacter dejongeii as well as all other species of Prosthecobacter is quite high at 91%, relative to the similarity of members of both these genera to deeper-branching species of verrucomicrobia such as Victivallis vadensis (76% in each case) or Opitutus terrae (80%). This should be more compatible with a late lateral gene transfer of tubulin genes into Prosthecobacter species than an ancient gene duplication in a verrucomicrobial ancestor (since retention of tubulin in both members of a resulting lineage or clade within verrucomicrobia would be expected if inherited vertically from a common ancestor).

Lateral transfer of tubulin genes seems more likely than transfer of FtsZ genes since a whole dcw cluster would have to be transferred. Loss and replacement of a complete multi-gene cell division mechanism necessary for viability of a cell population seems an unlikely scenario in case of one incorporating either FtsZ or tubulin, but the similarity of ancestral FtZs and tubulins could have meant such ancestral proteins could have been easily interchangeable within a common gene organization. Otherwise a version of the 'complexity hypothesis' [20]relating to cell division as a complex system of interacting proteins suggests that it would be difficult to replace FtsZ within a dcw cluster with a more evolved tubulin. From existing draft genome sequence, it appears that the significant gene for the FtsA protein important for interaction with FtsZ is missing from P. dejongeii, though genes of the dcw cluster concerned with peptidoglycan synthesis such as murD are present. P. dejongeii also seems to have a gene for FtsW, known to interact with peptidoglycan synthesis proteins[21]. Significantly, the tubulin genes in this species are organized in an operon along with a kinesin-like gene, suggesting they are not a part of the dcw cluster [13]. Co-expression of btuba and btubb in E. coli results in either prominent rods running along the cell length or loose spirals so this suggest that these proteins by themselves may be able to form cytoskeletal filaments in vivo [14].

Tubulin genes may be found in other verrucomicrobia branching more deeply than Prosthecobacter species within the verrucomicrobia. This is suggested by the report via anti-tubulin antibodies of tubulin in the 'epixenosomes', verrucomicrobial symbionts of marine ciliate protozoan Euplotidium [22]. Epixenosomes are members of subdivision 4 of the phylum Verrucomicrobia [23,24] along with the cultured Opitutus terrae. The distribution of genes for both tubulins and FtsZ must be determined in a wider selection of verrucomicrobia among all 5 subdivisions distinguished[25,26], e.g. cultured members of genera Opitutus and Chthoniobacter, in order to determine an optimal basis for further investigations of their evolution via data from genomics and cell biology. Since genome data for Verrucomicrobium spinosum and Prosthecobacter dejongeii is only in draft form so far, there is a possibility that either or both of these species possess both FtsZ and tubulin, and this should be confirmed or refuted by annotation of completed genomes for these species.

Discovery of a verrucomicrobial FtsZ in at least one genus and species combined with our existing knowledge of a tubulin in another verrucomicrobial genus reveals that two markedly different members of the tubulin-FtsZ cytoskeletal protein family occur within the same clade of the same bacterial phylum. This result may be of potential significance for our understanding of eukaryote and Bacterial cytoskeleton evolution, since such occurrence may suggest new models for cytoskeletal protein evolution e.g. derivation of both FtsZ and tubulin from an ancestral protein or proteins present in a Bacterial ancestor, or lateral transfer of cytoskeletal genes between domains at early stages in their evolution. Members of the phylum Verrucomicrobia may hold a key to the ancient molecular evolutionary stages by which tubulins and FtsZ's diverged and differentiated, or alternatively, the trace of an early event in the gene transfer network between prokaryotes and eukaryotes which may have preserved an early 'snapshot' of the evolution of eukaryote cytoskeletal proteins. Such models may be testable via a combination of further genomic and bioinformatic analysis of existing and newly discovered verrucomicrobia.

Methods

Bacterial strains and culture

Cultures of Verrucomicrobium spinosum DSM 4136 were grown aerobically at room temperature in modified medium B agar [27].

DNA extraction

DNA was extracted from Verrucomicrobium spinosum cultures by using the Instagene matrix (Bio-Rad).

Amplification of verftsz

The verftsz gene was amplified from single V. spinosum colonies via PCR. PCR primers used for the PCR (5' – 3') were as follows: AGTATTCCAGTGTAACCCG (forward) and GTCTCTTGAAGTGAAGGCTC (reverse). PCR was carried out with an initial denaturation at 96°C for 3 mins, followed by 32 cycles of 94°C for 1 min 30 sec, annealing at 58°C for 1 min, and extension at 72°C for 1 min 30 sec followed by a final extension step at 72°C for 10 mins. Primers used for sequencing were the same as for PCR with the addition of two internal sequencing primers (5' – 3') CTCCTTTGAGGGGCGTCGT (forward) and TGTCCCCTTCATCTTCCTCA (reverse).

Sequence analysis

The putative protein hereby termed VerFtsZ was analysed for signature motifs using the program FingerPRINTScan [28,29]. Gene family identification was done through Gene Family Identification Network Design (GeneFIND) [30]. Secondary structure of putative sequences was determined using the three-dimensional molecular structure viewer program Cn3D 4.1 [31] by structural homology matching against 1FSZ, the FtsZ structure of Methanococcus jannaschii [9]in the Protein Data Bank at NCBI.

Conserved region analysis

Regions of conservation such as the core and C-terminal regions were analysed based on a published analysis of FtsZ [4], and an alignment of VerFtsZ against published reference sequences of Bacterial amino acid sequences was performed using CLUSTALX. V. 1.8 [32]

Phylogenetic analysis

Alignment of VerFtsZ against reference sequences of Bacterial FtsZs was performed using CLUSTALX. V. 1.8 [32]. Optimization of the alignment i.e removal of variable spacer region and alignment of C-terminal region was performed using GeneDoc [33] The Modelgenerator [34] was used to obtain the model and parameters for the likelihood analysis for the alignment of VerFtsZ and FtsZ. Modelgenerator selected WAG + G as the best model for the maximum likelihood analyses based on the Akaike Information Criterion 1, Akaike Information Criterion 2, and Bayesian Information Criterion. A maximum likelihood tree was generated using TreeFinder [17]. Bootstrap analysis using 1,000 replicates with 4 gamma categories under the WAG substitution matrix [35] under the assumption of gamma-distributed, substitution rate variation [36] was performed on the tree with the highest likelihood score. Similarity values were calculated within PHYLIP Protdist advanced options within Institut Pasteur's Software for Biology site [37].

In silico analysis

Homology search

Gene searches utilized the Verrucomicrobium spinosum genome made available for public release by TIGR (The Institute for Genomic Research, Rockville, Maryland). Searches for the proteins of the dcw gene cluster (mraW, mraY, mraZ, murB, murC, murD, murE, murF, murG, FtsA, FtsI, FtsL, FtsQ, FtsW, FtsZ and ddlB) were conducted using query amino acid sequences from Escherichia coli and the similarity search function (tblastn) at The Institute of Genome Research (TIGR).

Authors' contributions

BY carried out the bioinformatic and phylogenetic analyses and designed PCR primers, and FFL participated in the phylogenetic analysis. BO carried out gene retrieval and sequencing from V. spinosum, JAF conceived of the study, participated in its design and coordination, and drafted the manuscript with BY, and JTS supplied V. spinosum cultures and sequence data on P. dejongeii. All authors read and approved the manuscript.

Acknowledgements

Research in the laboratory of JAF on verrucomicrobia is supported by Australian Research Council. Some preliminary sequence data was obtained from The Institute for Genomic Research (TIGR) through its website [38]. Sequencing of Verrucomicrobium spinosum DSM 4136 producing that draft genome was accomplished by TIGR with support from NSF (National Science Foundation).

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