Multiple transcripts of rh1 occur in the Australian lungfish retina. A) 3' RACE PCR products from lungfish rh1 cDNA. Arrows point to two potential transcripts differing in size in lane 2 (a third, faint band is also visible above these two but was not successfully sequenced), while lane 3 contained no template cDNA. The vertical grey line indicates where an irrelevant region of gel was removed using Photoshop 6.0 (Adobe). B) The 3' UTR of lungfish rh1. Two sequenced polyA signals are present (bold and italicised), both of which can induce polyadenylation producing two differently sized Rh1 transcripts.
Bailes et al. BMC Evolutionary Biology 2007 7:200 doi:10.1186/1471-2148-7-200