Figure 3.

RT-PCR using primer pairs S1.f2/N2.r2 and N1.f/N2.r2 across a range of different tissues. K = kidney, G = gut, H = heart, Li = liver, Lu = lung, S = spleen, B = brain, T = testis, O = ovary. For the N1.f/N2.r2 primer pair, reactions were performed with (+) and without (-) reverse transcriptase. The absence of a band in the – controls demonstrates that the larger unspliced products are true transcripts and not due to DNA contamination.