Expression of Ce-GnRHR. (A). Worm (N2) homogenates were immunoblotted with an affinity purified polyclonal antibody against Ce-GnRHR. The antibody recognized a 46-kDa band, the calculated molecular weight of the GnRHR1 orthologue. The secondary antibody alone did not show immunoreactivity. The molecular weight markers are indicated in kDa on the left. (B). Immunohistochemical localization of Ce-GnRHR was performed on eggs after freeze fracture using a monoclonal antibody against human GnRHR1 (above) or an affinity purified polyclonal antibody against Ce-GnRHR (below). Eggs were similarly stained by both antibodies. No immunoreactivity was detected in eggs probed with the secondary antibody alone. Scale: eggs – 50 μm.
Vadakkadath Meethal et al. BMC Evolutionary Biology 2006 6:103 doi:10.1186/1471-2148-6-103