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Open Access Research article

Genetic species identification and population structure of Halophila (Hydrocharitaceae) from the Western Pacific to the Eastern Indian Ocean

Vy X Nguyen12, Matsapume Detcharoen3, Piyalap Tuntiprapas4, U Soe-Htun5, Japar B Sidik6, Muta Z Harah7, Anchana Prathep3 and Jutta Papenbrock1*

Author Affiliations

1 Institute of Botany, Leibniz University Hannover, Herrenhäuserstr. 2, D-30419 Hannover, Germany

2 Department of Marine Botan, Institute of Oceanography, Vietnam Academy of Science and Technology, 01 Cau Da, Nha Trang City, Vietnam

3 Department of Biology, Faculty of Science, Prince of Songkla University, HatYai, Songkhla 90112, Thailand

4 Seaweed and Seagrass Research Unit, Excellence Centre for Biodiversity of Peninsular Thailand, Faculty of Science, Prince of Songkla University, HaiYai, Songkhla 90112, Thailand

5 Department of Marine Science, Mawlamyine University, Mawlamyine, Myanmar

6 Department of Animal Science and Fishery, Faculty of Agriculture and Food Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, Nyabau Rd, P.O. Box 386, 97008 Bintulu, Sarawak, Malaysia

7 Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia

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BMC Evolutionary Biology 2014, 14:92  doi:10.1186/1471-2148-14-92

Published: 30 April 2014

Abstract

Background

The Indo-Pacific region has the largest number of seagrass species worldwide and this region is considered as the origin of the Hydrocharitaceae. Halophila ovalis and its closely-related species belonging to the Hydrocharitaceae are well-known as a complex taxonomic challenge mainly due to their high morphological plasticity. The relationship of genetic differentiation and geographic barriers of H. ovalis radiation was not much studied in this region. Are there misidentifications between H. ovalis and its closely related species? Does any taxonomic uncertainty among different populations of H. ovalis persist? Is there any genetic differentiation among populations in the Western Pacific and the Eastern Indian Ocean, which are separated by the Thai-Malay peninsula? Genetic markers can be used to characterize and identify individuals or species and will be used to answer these questions.

Results

Phylogenetic analyses of the nuclear ribosomal internal transcribed spacer region based on materials collected from 17 populations in the Western Pacific and the Eastern Indian Ocean showed that some specimens identified as H. ovalis belonged to the H. major clade, also supported by morphological data. Evolutionary divergence between the two clades is between 0.033 and 0.038, much higher than the evolutionary divergence among H. ovalis populations. Eight haplotypes were found; none of the haplotypes from the Western Pacific is found in India and vice versa. Analysis of genetic diversity based on microsatellite analysis revealed that the genetic diversity in the Western Pacific is higher than in the Eastern Indian Ocean. The unrooted neighbor-joining tree among 14 populations from the Western Pacific and the Eastern Indian Ocean showed six groups. The Mantel test results revealed a significant correlation between genetic and geographic distances among populations. Results from band-based and allele frequency-based approaches from Amplified Fragment Length Polymorphism showed that all samples collected from both sides of the Thai-Malay peninsula were clustered into two clades: Gulf of Thailand and Andaman Sea.

Conclusions

Our study documented the new records of H. major for Malaysia and Myanmar. The study also revealed that the Thai-Malay peninsula is a geographic barrier between H. ovalis populations in the Western Pacific and the Eastern Indian Ocean.

Keywords:
Eastern Indian Ocean; Evolution; Genetic distance; Halophila ovalis; Western Pacific Ocean