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Revealing the maternal demographic history of Panthera leo using ancient DNA and a spatially explicit genealogical analysis

Ross Barnett19*, Nobuyuki Yamaguchi2, Beth Shapiro3, Simon YW Ho4, Ian Barnes5, Richard Sabin6, Lars Werdelin7, Jacques Cuisin8 and Greger Larson1

Author Affiliations

1 Durham Evolution and Ancient DNA, Department of Archaeology, Durham University, Durham DH1 3LE, UK

2 Department of Biological and Environmental Sciences, Qatar University, Doha, Qatar

3 Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA 95064, USA

4 School of Biological Sciences, University of Sydney, Sydney, NSW 2006, Australia

5 School of Biological Sciences, Royal Holloway University of London, Egham, Surrey TW20 0EX, UK

6 Department of Life Science, Natural History Museum, Cromwell Road, London SW7 5BD, UK

7 Department of Palaeobiology, Swedish Museum of Natural History, Box 50007, Stockholm SE-10405, Sweden

8 Muséum National d’Histoire Naturelle, 54 Rue Cuvier, Paris 75005, France

9 Current Address: Centre for GeoGenetics, København Universitet, The Natural History Museum Øster Voldgade, Copenhagen 5-7 DK-1350, Denmark

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BMC Evolutionary Biology 2014, 14:70  doi:10.1186/1471-2148-14-70

Published: 2 April 2014

Additional files

Additional file 1: Figure S1:

Schematic of contig overlaps indicating their amplification positions along 1140bp of cytochrome b.

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Additional file 2: Figure S2:

Neighbour-joining trees produced with PAUP* [72] of individual amplicons produced in this study, compared to known cymt (AF053040 & AF053018) and numt (AF053053 & AF053054) sequences from tiger [73].

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Additional file 3: Table S1:

List of all Genbank accessible comparative sequences and relevant information.

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Additional file 4: Table S2:

List of all primers used and their annealing temperatures and lengths.

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Additional file 5: Table S3:

Table of number of independent PCRs sequenced for each region. Cells with an outlined border indicate regions containing unique mutations not found in other lions. Where 2 cells share the same outlined border indicates mutation identified in region of PCR overlap and present in both contigs.

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