Experimental design. Outbred F1 progeny were derived from the ten DGRP lines using a ring crossing design, such that each line contributed a mother for one experimental genotype and a father for another. Virgin F1 females were exposed to males 24 hours prior to infection. Flies were infected with P. rettgeri. After infection flies were split into two groups: those for measuring bacterial load and those for measuring survival and fecundity. All vials were transferred daily and maintained at a density of 2–3 females. Each day a subsample of flies were removed for the destructive bacterial load assay. Daily mortality was recorded for the survival and fecundity vials, and the vials were saved to count the number of eventually emerging adult offspring. All of this was performed on the two diets that differed in the level of glucose.
Howick and Lazzaro BMC Evolutionary Biology 2014 14:56 doi:10.1186/1471-2148-14-56