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Open Access Highly Accessed Methodology article

Evolution of MHC class I genes in the endangered loggerhead sea turtle (Caretta caretta) revealed by 454 amplicon sequencing

Victor A Stiebens1, Sonia E Merino2, Frédéric J J Chain3 and Christophe Eizaguirre13*

Author Affiliations

1 Department of Evolutionary Ecology of Marine Fishes, GEOMAR|Helmholtz Center for Ocean Research, Kiel, 24105, Germany

2 National Institute for Development and Fishery (INDP), Mindelo, Cape Verde

3 Department of Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Ploen, 24306, Germany

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BMC Evolutionary Biology 2013, 13:95  doi:10.1186/1471-2148-13-95

Published: 30 April 2013

Additional files

Additional file 1: Figure S1:

Neighbor-joining tree of the mtDNA control region. All sequences have been deposited on Archie Carr Centre for Sea Turtle Research ( webcite). Document 2 Amino acid alignment of loggerhead turtle MHC class I alleles. Dots indicate identity with the loggerhead Cc*0 sequence. Document 3 Table summarizing the genotyping of 7 turtles using two different methods: cloning/sequencing vs. 454 sequencing. Allele identities are given together with the number of clones picked and sequenced for each individual. Row in bold shows a discrepancy between cloning and 454 sequencing. $indicates a posteriori screen.

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