Table 2

Major PEPCK amino acid variation shared by Colias species
Taxon Codons
335 503 629
n p aa n p aa n p aa
CEU 20 0.56 Gly 7 0.19 Asp 18 0.5 Ile
16 0.44 Ser 29 0.81 Glu 18 0.5 Val
CME 15 0.42 Gly 4 0.11 Asp 6 0.17 Ile
21 0.58 Ser 32 0.89 Glu 30 0.83 Val
x*− 1.18 0.98 3.00
P 0.24 0.33 0.003

CEU, C. eurytheme; CME, C. meadii; n, variant counts; p, variant frequencies; aa, amino acids; x*, test parameter (normal deviate) for Goldstein’s exact binomial test for frequency difference [26]; P, probability of as much or more difference between samples by chance. Given n = 3 codons to test simultaneously, the corrected (Dunn-Sidak) minimum significance level α′ = [1 – (1– α)1/n = 0.017 [27].

Watt et al.

Watt et al. BMC Evolutionary Biology 2013 13:9   doi:10.1186/1471-2148-13-9

Open Data