Additional file 3:.

Tests of linkage disequilibrium among varying amino acid sites. Abbreviations: amino acids, standard one-letter codes; obs, observed; exp, expected; D, linkage disequilibrium = pX1pX4 – pX2pX3; GYates(1), G statistic with Yates’ correction and 1 degree of freedom; Fisher’s exact, test of that name [ref]; P, probability of this or more extreme result by chance alone. a) tests of sites polymorphic in both Colias species. Site variant frequencies in main text Table 2. Correction of significance threshold for multiple tests by Dunn-Sidak method [ref], 6 tests, α = 0.0085. No linkage disequilibria are significant. b) tests of Arg/Lys 11, polymorphic only in mitochondrial 5exon of C. eurytheme, and the other polymorphic sites in that species. Site frequencies at site 11: Arg 0.556, Lys 0.444; other site frequencies as above. 3 tests, α = 0.017. No linkage disequilibria are significant.

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Watt et al. BMC Evolutionary Biology 2013 13:9   doi:10.1186/1471-2148-13-9