Additional file 1.
Amplifying and sequencing primers for study of Colias PEPCK. Amplifying primers are of course used for sequencing as well. ″5end″ and ″3end″ primers are located in 5′- and 3′-untranslated regions (UTRs). S and A denote sense and antisense primer directions, respectively. Overlapping gene subsets amplified for sequencing: MVY-S-5end and MLH-S2-5end / A1005 (one each per individual); S511C / A1519C; and S1487 / A3end. Primer numbers denote primers’ 3′-terminal nucleotide positions in the gene.
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Watt et al. BMC Evolutionary Biology 2013 13:9 doi:10.1186/1471-2148-13-9