Additional file 1.

Amplifying and sequencing primers for study of Colias PEPCK. Amplifying primers are of course used for sequencing as well. ″5end″ and ″3end″ primers are located in 5- and 3-untranslated regions (UTRs). S and A denote sense and antisense primer directions, respectively. Overlapping gene subsets amplified for sequencing: MVY-S-5end and MLH-S2-5end / A1005 (one each per individual); S511C / A1519C; and S1487 / A3end. Primer numbers denote primers’ 3-terminal nucleotide positions in the gene.

Format: DOCX Size: 12KB Download file

Watt et al. BMC Evolutionary Biology 2013 13:9   doi:10.1186/1471-2148-13-9