Table 1

Functional characterisation of Northern pike Elovl5 elongase, and its role in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA)
PUFA substrate Product Conversion (%) Activity
E. lucius S. salar S. salar
Elovl5 Elovl5a Elovl5b
18:3n-3* 20:3n-3 39 - - C18 → 20
22:3n-3 4 - - C20 → 22
Total 43 - -
18:4n-3* 20:4n-3 67 56 58 C18 → 20
22:4n-3 5 7 4 C20 → 22
Total 72 63 62
20:5n-3* 22:5n-3 67 36 68 C20 → 22
24:5n-3 5 1 1 C22 → 24
Total 72 37 69
22:5n-3* 24:5n-3 4 1 1 C22 → 24
18:2n-6* 20:2n-6 26 - - C18 → 20
22:2n-6 2 - - C20 → 22
Total 28 - -
18:3n-6* 20:3n-6 67 43 65 C18 → 20
22:3n-6 7 5 6 C20 → 22
Total 74 48 71
20:4n-6* 22:4n-6 35 23 48 C20 → 22
24:4n-6 1 1 1 C22 → 24
Total 36 24 49
22:4n-6* 24:4n-6 1 1 1 C22 → 24

Data are expressed as percentage of PUFA converted to elongated products at each step of the pathway and overall when yeast were transformed with pYES2-Elovl5 construct and incubated in presence of C18, C20 or C22 substrates. Atlantic salmon data extracted from [25].

Carmona-Antoñanzas et al.

Carmona-Antoñanzas et al. BMC Evolutionary Biology 2013 13:85   doi:10.1186/1471-2148-13-85

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