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Open Access Research article

Mitochondrial lineage sorting in action – historical biogeography of the Hyles euphorbiae complex (Sphingidae, Lepidoptera) in Italy

Michael B Mende12* and Anna K Hundsdoerfer12

Author Affiliations

1 Museum für Tierkunde, Senckenberg Naturhistorische Sammlungen Dresden, Königsbrücker Landstr. 159, Dresden, 01109, Germany

2 Biodiversität und Klima Forschungszentrum (BiK-F), Senckenberganlage 25, Frankfurt am Main, 60325, Germany

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BMC Evolutionary Biology 2013, 13:83  doi:10.1186/1471-2148-13-83

Published: 18 April 2013

Additional files

Additional file 1: Table S1:

Origin and other information about the historical samples included in this study. MTD-#: voucher number in the invertebrate tissue catalogue of the Museum für Tierkunde, Dresden; Museum: source of the sample (abbreviation according to Table 1); Locality: data in square brackets completed by inference; Coordinates: inferred from locality data on label, italic coordinates indicate imprecise locality; Mt-lineage: mitochondrial lineage according to Hundsdoerfer et al.[31], BL = no successful PCR of fragment H, HL = no successful PCR of fragment B, L = successful PCR of fragment L only, --- = no successful PCR of any fragment; Extr: 2x = repeated DNA extraction; Variety: grentzenbergi = reddish forewing pattern variety.

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Additional file 2: Table S2:

Variable sites of the three amplicons used in this study for the Mediterranean lineages of the HEC. Consensus sequences for each Mediterranean HEC lineage of all individuals from Hundsdoerfer et al. [31] were reduced to variable sites of the three fragments B, H, L. Character states for the positive control outgroup H. livornica are reported for comparison. Positions are numbered according to the 2284 bp alignment of COI/II genes of Hundsdoerfer et al. [31]; fragment B: position 89–365, fragment H: 1094–1373, fragment L: 1909–2145. Round brackets indicate that not all individuals of the lineage bear the substitution; lowercases indicate rare substitutions in only few or single specimens.

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