Open Access Highly Accessed Research article

Parallel evolution of senescence in annual fishes in response to extrinsic mortality

Eva Terzibasi Tozzini12, Alexander Dorn1, Enoch Ng’oma1, Matej Polačik3, Radim Blažek3, Kathrin Reichwald1, Andreas Petzold1, Brian Watters4, Martin Reichard3 and Alessandro Cellerino12*

Author Affiliations

1 Fritz-Lipmann Institute for Age Research, Leibniz Institute, Jena, Germany

2 Scuola Normale Superiore, Pisa, Italy

3 Institute of Vertebrate Biology Academy of Sciences of the Czech Republic, Brno, Czech Republic

4 36141 Parkwood Drive, Nanaimo, British Columbia, Canada

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BMC Evolutionary Biology 2013, 13:77  doi:10.1186/1471-2148-13-77

Published: 3 April 2013

Additional files

Additional file 1: Table S2:

Life span and sample size of the captive populations of the N. furzeri/N. kuhntae clade used for the study.

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Additional file 2: Table S3:

Pairwise Log-rank statistics of survivorship for all populations of the N. furzeri/N. kunthae clade in the study. FUR = N. furzeri, KUN = N. Kuhntae.

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Additional file 3: Figure S1:

Age-dependent mortality in N. furziri and N. kuhntae. The lines report smoothed mortality curves for the two species. Each point represents the average of four consecutive weeks. In order to allow exponential fit, zero values were substituted with 0.001.

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Additional file 4:Table S4:

Life span and sample size of the captive populations of the N. pienaari/N. rachovii clade used for the study.

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Additional file 5: Table S5:

Pairwise Log-rank statistics of survivorship for all populations of the N. pienaari/N. rachovii clade used for the study. PIE = N. pienaari, RAC = N. Rachovii.

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Additional file 6: Table S6:

Kruskall-Wallis ANOVA of lipofuscin accumulation in the liver, pair-wise comparisons for all the populations of the N. furzeri/N. kunthae clade in the study. FUR = N. furzeri, KUN = N. Kuhntae.

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Additional file 7: Table S7:

Kruskall-Wallis ANOVA of lipofuscin accumulation in the liver, pair-wise comparisons for all the populations of the N. pienaari/N. rachovii clade used for the study. PIE = N. pienaari, RAC = N. rachovii.

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Additional file 8: Table S8:

Kruskall-Wallis ANOVA of lipofuscin accumulation in the brain, pair-wise comparisons for all the populations of the N. furzeri/N. kunthae clade in the study. FUR = N. furzeri, KUN = N. kuhntae.

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Additional file 9: Table S9:

Kruskall-Wallis ANOVA of lipofuscin accumulation in the brain, pair-wise comparisons for all the populations of the N. pienaari/N. rachovii clade used for the study. PIE = N. pienaari, RAC = N. rachovii.

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Additional file 10: Table S10:

Microsatellite polymorphisms in the four studied species - Microsatellites were amplified using PCR on genomic DNA and microsatellites ID are described in Valenzano et al. (2009). For each of the two alleles, the length of the amplified fragment is reported. For homozygous loci, both these values are identical. One individual for each species was analyzed, n.a. indicates no amplification.

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Additional file 11: Table S11:

Sequence divergence between N. furzeri and N. kuhntae measured at 115 loci - Column 1 reports the gene name, Column 2 reports the length of the coding sequence in N. furzeri, column 3 reports the length of the alignment between the two ortholog sequences, column 4 reports the percentage of the N. furzeri coding sequence covered by the alignment, column 5 reports the ID of the N. furzeri transcript, column 6 reports the ID of the N. kunthae genomic contig, column 7 reports the number of sites of possible non-synonimous substitution, column 8 reports the frequency of non-synonimous substituions per site, column 9 reports the number of sites of possible synonimous substitution (dN), column 10 reports the frequency of synonimous substituions per site (dS), column 10 reports the dN/dS ratio, column 11 reports the percentage of aminoacidic sequence identity in the alignment, column 12 reports the percentage of nucleotide sequence identity in the alignment, column 12 reports the number of nonsynonimous sequence variations and column 13 the number of synonymous sequence variations. The last two rows report the median of all columns and the total for columns 2, 3, 6, 8, 13 and 14.

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Additional file 12: Table S1:

The number of dataloggers which were still submerged (water) and loggers exposed on dry bottom (dry) recovered at each region.

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