Table 1

Activation kinetics of ADP-Glc PPases fromAnabaena,O. tauri, and potato tuber
Enzyme v0(U mg-1) Activator A0.5(mM) Activation fold Net activation fold Specificity constant (mM-1)
Anabaena 0.77 3-PGA 0.075 5.5 4.5 60
FBP 0.30 1.2 0.2 0.7
Fru6P 1.6 2.5 1.5 1.0
Glc6P 0.40 1.4 0.4 1.0
OtaS/OtaL 0.89 3-PGA 0.54 33 32 59
FBP 1.1 7.0 6.0 5.5
Fru6P 15 2.7 1.7 0.1
Glc6P 75 17 16 0.2
StuS/StuL 0.27 3-PGA 0.054 68 67 1241
FBP 0.84 6.0 5.0 6.0
Fru6P 1.5 57 56 36
Glc6P 1.8 29 28 15
OtaS 0.25 3-PGA 1.2 10 9.0 8.0
FBP 1.1 9.3 8.3 7.6
Fru6P 1.9 3.9 2.9 1.6
Glc6P 10 5.5 4.5 0.4
OtaSD148A/OtaL 0.57 3-PGA 0.003 7.0 6.0 2000
FBP 8.2 5.0 4.0 0.5
Fru6P 0.74 5.0 4.0 5.4
Glc6P 65 26 25 0.4
OtaS/OtaLD171A 0.12 3-PGA 0.067 5.1 4.1 61
FBP 1.5 5.9 4.9 3.3
Fru6P 2.9 5.0 4.0 1.4
Glc6P 3.1 3.4 2.4 0.8

Activity of the purified enzymes was assayed in the ADP-Glc synthesis direction. Kinetic parameters were calculated as described under “Methods”. For each enzyme and each activator the Vmax can be calculated as v0 times the Activation Fold. StuS and StuL are the S and L subunits of potato tuber, and OtaS and OtaL the S and L subunits of O. tauri, respectively.

Kuhn et al.

Kuhn et al. BMC Evolutionary Biology 2013 13:51   doi:10.1186/1471-2148-13-51

Open Data