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The ancestral activation promiscuity of ADP-glucose pyrophosphorylases from oxygenic photosynthetic organisms

Misty L Kuhn13, Carlos M Figueroa12, Alberto A Iglesias2 and Miguel A Ballicora1*

  • * Corresponding author: Miguel A Ballicora

  • † Equal contributors

Author Affiliations

1 Department of Chemistry and Biochemistry, Loyola University Chicago, 1032 W. Sheridan Rd, Chicago, IL, 60660, USA

2 Instituto de Agrobiotecnología del Litoral (UNL-CONICET), Facultad de Bioquímica y Ciencias Biológicas (UNL), Ciudad Universitaria, Santa Fe, S3000ZAA, Argentina

3 Present address: Department of Molecular Pharmacology and Biological Chemistry, Northwestern University Feinberg School of Medicine, 303 E. Chicago Avenue, Chicago, IL, 60611, USA

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BMC Evolutionary Biology 2013, 13:51  doi:10.1186/1471-2148-13-51

Published: 21 February 2013

Additional files

Additional file 1: Figure S1:

Sequence alignment used for the phylogenetic tree. The alignment was initially performed with the ClustalW server and manually refined to introduce insertions and deletions in loop regions (based in the crystal structure of the potato tuber ADP-Glc PPase), as described under “Methods”. During the manual refinement of the alignment, 10 sequences were removed prior to tree reconstruction. Sequences 83, 85, 86, 93, 202, and 205 were removed due to their similarity with the A. thaliana aps2, which has no detectable activity in vitro[6]. Sequences 65, 78, and 79 were removed because they have an insertion in a region predicted as a β-sheet and the sequences have not been subjected to the NCBI final revision. Sequence 40 was removed because its C-terminal region was incomplete. Residues are colored based on their chemical properties.

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Additional file 2: Table S1:

Data of sequences used for the phylogenetic tree. The table contains the number used for each sequence, the corresponding NCBI accession number and annotation, the name of the organism, and the taxonomic group. Colors used are the same as in the phylogenetic tree (Figure 1).

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