Table 2

Nucleotide sequences for the specific primers and MGB™ probes, melting temperatures (Tm), fragment lengths and PCR efficiencies
Target Primer/Probe Nucleotide sequence Tm Fragment length PCR efficiency
Hsp70 CY-A Hsp70cyA_rt_F 5-TCA GGA GCT GAT GAC AAA CCA A-3 59°C 74 bp 90%
Hsp70cyA_rt_R 5-ATC TCC TCT GGA TGG AAT TTC TTG-3 59°C
Hsp70cyA_MGB_VIC 5-TGT GGT TAA GTA TAA AGG TGA G-3 67°C
Hsp70 CY-B Hsp70cyB_rt_F4 5-TGC ATA TGG TGC TGC TGT TTA AG-3 59°C 133 bp 89%
Hsp70cyB_rt_R2 5-TAA TAC ACT CAT TAC ACC ACC TGC AG-3 59°C
Hsp70cyB_MGB_VIC 5-CCA CTT TCA TTA GGA ATT-3 68°C
Hsp70 ER-A Hsp70ERA_rt_F 5-GAA GTT CTC ACT AGA GCC AGA TTC G-3 59°C 76 bp 93%
Hsp70ERA_rt_R 5-CTG ATT ACA TGG GAC CAG TTG TCT-3 58°C
Hsp70ERA_MGB_VIC 5-ACT CAA TTC AGA TCT C-3 68°C
Hsp70 ER-B Hsp70ERB_rt_F 5-GTT GAG AAG GGA ACC TAA TAA AAG GTC-3 59°C 98 bp 92%
Hsp70ERB_rt_R 5-GTC CCA AAT ATC CTT CAG CAA TCT-3 59°C
Hsp70ERB_MGB_VIC 5-CAG AGG AGA TTA GTG CCA TG-3 68°C
Hsp70 MT Hsp70mt_rt_F 5-ACC AGA ATG CCA AAA GTC CAA-3 58°C 76 bp 91%
Hsp70mt_rt_R 5-CAT CTG GGT TGA CTG ACT TGT TG-3 58°C
Hsp70mt_MGB_VIC 5-AAG ATT TAT TCG ACA AAC CA-3 67°C
GAPDH GAPDH_rt_F 5-GCT GCC AAG GCT GTT GGT-3 58°C 75 bp 92%
GAPDH_rt_R 5-TGT TGG AAC TCT GAA GGC CAT AC-3 59°C
GAPDH_MGB_FAM 5-TCC CAG AAA TCA AAG-3 67°C
EF-1a EF1a_rtF2 5-TTG ATG CCC CAG GAC ATA GAG-3 59°C 95 bp 94%
EF1a_rtR2 5-TCC TGC TGG TGA GGC AAT C-3 60°C
EF1a_MGB_FAM 5-TAC AGG AAC ATC ATA AGC-3 68°C

Krenek et al.

Krenek et al. BMC Evolutionary Biology 2013 13:49   doi:10.1186/1471-2148-13-49

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