Relative mRNA transcript induction of the five P. caudatum Hsp70-groups after heat shock. Expression ratios were assessed by RT-qPCR using 28°C as control treatment and two-hour incubation at 34°C as heat-shock treatment. Transcript levels of the Hsp70-groups CY-A, CY-B, ER-A, ER-B and MT were normalised to the reference genes GAPDH and EF-1a. Relative expression ratios (fold-change) were calculated using the PCR-efficiency based method in the programme REST 2009. Results are illustrated as Whisker-box plots where the dotted line represents the sample median (n = 5) and the box area encompasses 50% of all observations. The grey dashed line defines the value of no change in relative expression.
Krenek et al. BMC Evolutionary Biology 2013 13:49 doi:10.1186/1471-2148-13-49