Phylogenetic reconstruction of homologous hsp70 sequences from different pro- and eukaryotes. The alignment was based on a comprehensive amino acid alignment containing 97 homologs that show the typical Hsp70 family signatures. Sequences obtained within this study are shown in boldface. The alignment length was constricted to 489 amino acids, including gaps. The illustrated tree is based on the Maximum-Likelihood calculations using the LG+I+Γ protein evolution model with 1,000 rapid bootstrap replicates. The Archaea DnaK sequences served as outgroup. Numbers at the nodes (occasionally indicated by an arrow) represent support values for the Maximum-Likelihood (above line) and Bayesian analysis (below line), respectively. The three major Hsp70-subfamilies—cytosol (CY), endoplasmic reticulum (ER) and mitochondria (MT)—are indicated by squared brackets to the right. Vertical bars to the right of the tree designate the five different Hsp70-groups (CY-A, CY-B, ER-A, ER-B, MT) detected in Paramecium. Highly heat inducible cytosol-type hsp70 genes (if known) are specified by an asterisk. Filled circles at the nodes represent ancient and intermediary gene duplication events, while filled triangles at the nodes point to speciation within Paramecium. Rounded brackets to the right exemplify evolutionary relationships among the Paramecium Hsp70 homologs and are defined in the upper left box frame. See Additional file 4: Figure S2 for the uncollapsed tree and its electronic version deposited in TreeBASE under accession number TB2:S13746.
Krenek et al. BMC Evolutionary Biology 2013 13:49 doi:10.1186/1471-2148-13-49