ONPG analysis of AX4 cells expressing the lacZ gene under the prespore promoter cotB. Cells were developed alone or mixed at a 1:1 ratio with AX4 and chtB cells. Developmental time points (hours) are represented on the x-axis and beta-galactosidase activity on the y-axis. When AX4 [cotB/lacZ] are mixed with chtB cells, the β-galactosidase activity is lower than when mixed with AX4 (16, 20 and 24 hour time points, t-test, p<0.05, n=3).
Santorelli et al. BMC Evolutionary Biology 2013 13:4 doi:10.1186/1471-2148-13-4