The chtB gene is expressed during development and is completely silent in our mutant. RT-PCR reaction using RNA extracted during a time course (time points indicated in hours) shows that the chtB mRNA is observed only in AX4 during development. No mRNA is detected in the mutant chtB.
Santorelli et al. BMC Evolutionary Biology 2013 13:4 doi:10.1186/1471-2148-13-4